Irreversible Photobleaching of the Solution of Fluorescent Dyes. V. Photobleaching of Erythrosin

Photobleaching of Fluorescent Dyes in Polymer Films

Recently, optical data storage has been achieved via photobleaching of a fluorescent dye in co-extruded, multilayer, polymer films. Photobleaching, the decrease in fluorescence by exposure to light, can be caused by a number of different processes, including light-induced chemical reactions and thermal degradation. Although data has successfully been written using this method, the mechanisms co...

Nonlinear dynamics of absorption and photobleaching of dyes.

The celebrated Lambert-Beer law of light absorption in photochromic media is only valid at low intensities of incident light and low concentration of chromophore. Here we address the generic problem of photoabsorption dynamics, experimentally studying the case of azobenzene isomerization. We show that the nonlinear regime is very common and easy to achieve in many practical situations, especial...

A reducing and oxidizing system minimizes photobleaching and blinking of fluorescent dyes.

Beyond photobleaching, laser illumination unbinds fluorescent proteins.

Confocal and two-photon fluorescence microscopy techniques using genetically encoded fluorescent probes are widely used in cell biology. Beyond the common problems of photobleaching and phototoxicity, we present evidence that photounbinding also has the potential to compromise such methods, especially in quantitative studies. We show that laser intensities within excitation regimes typical for ...

analysis of reading comprehension needs of the students of paramedical studies: the case of the students of health information management (him)

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